经过测试的应用

Positive WB detected in mouse brain tissue, A549 cells

Positive IP detected in mouse brain tissue

Positive IHC detected in human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

APPLICATION DILUTION

Western Blot (WB) WB : 1:1000-1:8000

Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunohistochemistry (IHC) IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB See 4 publications below

IP See 1 publications below

产品信息

55129-1-AP targets Neurabin 2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description

Cited Applications WB, IP

Tested Reactivity Human, Mouse

Cited Reactivity Human, Mouse

Immunogen Peptide 种属同源性预测

Host / Isotype Rabbit / IgG

Class Polyclonal

Type Antibody

Full Name protein phosphatase 1, regulatory (inhibitor) subunit 9B

Synonyms Neurabin 2, Neurabin II, PPP1R6, PPP1R9, PPP1R9B, SPINO, Spinophilin, Spn

Calculated Molecular Weight 89 kDa

Observed Molecular Weight 120-130 kDa

GenBank Accession Number NM_032595

Gene Symbol Spinophilin/Neurabin 2

Gene ID (NCBI) 84687

RRID AB_10837358

Conjugate Unconjugated

Form Liquid

Purification Method Antigen affinity purification

UNIPROT ID Q96SB3

Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.