经过测试的应用

Positive WB detected in C2C12 cell, mouse brain tissue, C2C12 cells

Positive IHC detected in human prostate cancer tissue, mouse brain tissue, mouse skeletal muscle tissue, human cerebellum tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF-P detected in mouse brain tissue

Planning an IHC experiment? We recommend our IHCeasy ATP1A3 Ready-To-Use IHC Kit. ATP1A3 primary antibody included.

推荐稀释比

APPLICATION DILUTION

Western Blot (WB) WB : 1:500-1:3000

Immunohistochemistry (IHC) IHC : 1:50-1:500

Immunofluorescence (IF)-P IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB See 2 publications below

IHC See 2 publications below

产品信息

10868-1-AP targets ATP1A3 (C-terminal) in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description

Cited Applications WB, IHC

Tested Reactivity Human, Mouse, Rat

Cited Reactivity Human

Immunogen ATP1A3 (C-terminal) fusion protein Ag1313 种属同源性预测

Host / Isotype Rabbit / IgG

Class Polyclonal

Type Antibody

Full Name ATPase, Na+/K+ transporting, alpha 3 polypeptide

Synonyms ATP1A3 (middle), ATP1A3, EC:7.2.2.13, DYT12, ATP1A 3

Calculated Molecular Weight 113 kDa

Observed Molecular Weight 100-113 kDa

GenBank Accession Number BC015566

Gene Symbol ATP1A3

Gene ID (NCBI) 478

RRID AB_2060962

Conjugate Unconjugated

Form Liquid

Purification Method Antigen affinity purification

UNIPROT ID P13637

Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.