经过测试的应用

Positive WB detected in mouse brain tissue, U-251 cells, rat brain tissue

Positive IHC detected in mouse brain tissue, Alzheimer mouse

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF-P detected in rat brain tissue, mouse brain tissue

Positive IF-Fro detected in mouse brain tissue

Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

APPLICATION DILUTION

Western Blot (WB) WB : 1:2000-1:10000

Immunohistochemistry (IHC) IHC : 1:2500-1:10000

Immunofluorescence (IF)-P IF-P : 1:50-1:500

Immunofluorescence (IF)-FRO IF-FRO : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB See 163 publications below

IHC See 91 publications below

IF See 370 publications below

产品信息

16825-1-AP targets GFAP in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IF-Fro, ELISA Application Description

Cited Applications WB, IHC, IF

Tested Reactivity Human, Mouse, Rat

Cited Reactivity Human, Mouse, Rat, Pig, Rabbit, Monkey, Zebrafish, Hamster, Goat, Duck

Immunogen GFAP fusion protein Ag10423 种属同源性预测

Host / Isotype Rabbit / IgG

Class Polyclonal

Type Antibody

Full Name glial fibrillary acidic protein

Synonyms glial fibrillary acidic protein

Calculated Molecular Weight 432 aa, 50 kDa

Observed Molecular Weight 45-50 kDa

GenBank Accession Number BC013596

Gene Symbol GFAP

Gene ID (NCBI) 2670

RRID AB_2109646

Conjugate Unconjugated

Form Liquid

Purification Method Antigen affinity purification

UNIPROT ID P14136

Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.