经过测试的应用

Positive WB detected in HEK-293 cells, A549 cells, mouse skeletal muscle tissue, mouse liver tissue, mouse brain tissue, Hela cells, HepG cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue

Positive IP detected in MCF-7 cells

Positive IHC detected in human oesophagus cancer tissue, human skin cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF/ICC detected in HeLa cells

Planning an IHC experiment? We recommend our IHCeasy Histone-H3 Ready-To-Use IHC Kit. Histone-H3 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

APPLICATION DILUTION

Western Blot (WB) WB : 1:2000-1:16000

Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunohistochemistry (IHC) IHC : 1:50-1:500

Immunofluorescence (IF)/ICC IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO See 2 publications below

WB See 840 publications below

IHC See 1 publications below

IF See 10 publications below

IP See 1 publications below

CoIP See 1 publications below

ChIP See 7 publications below

产品信息

17168-1-AP targets Histone H3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description

Cited Applications WB, IHC, IF, IP, CoIP, ChIP

Tested Reactivity Human, Mouse, Rat

Cited Reactivity Human, Mouse, Rat, Pig, Monkey, Chicken, Goat, Fish, Arabidopsis, Yellow Catfish

Immunogen Histone H3 fusion protein Ag10644 种属同源性预测

Host / Isotype Rabbit / IgG

Class Polyclonal

Type Antibody

Full Name histone cluster 2, H3a

Synonyms HIST2H3A, H3-clustered histone 15, H3-clustered histone 14, H3-clustered histone 13, H3C15

Calculated Molecular Weight 136 aa, 15 kDa

Observed Molecular Weight 15-17 kDa

GenBank Accession Number BC015544

Gene Symbol Histone H3

Gene ID (NCBI) 333932

RRID AB_2716755

Conjugate Unconjugated

Form Liquid

Purification Method Antigen affinity purification

UNIPROT ID Q71DI3

Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.